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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
12/11/2015 |
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Actualizado : |
28/10/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
BENÍTEZ-GALEANO, M.J.; RUBIO, L.; BERTALMIO, A.; MAESO, D.; RIVAS, F.; COLINA, R. |
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Afiliación : |
MARÍA JOSÉ BENÍTEZ-GALEANO, Universidad de la República (UdelaR)/ Centro Universitario Regional Noroeste, Salto (CENUR); LETICIA PAOLA RUBIO CATTANI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ANA MARIA BERTALMIO CASARIEGO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; DIEGO CESAR MAESO TOZZI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; CARLOS FERNANDO RIVAS GRELA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; RODNEY COLINA, Universidad de la República (UdelaR)/ Centro Universitario Regional Noroeste, Salto (CENUR). |
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Título : |
Phylogenetic studies of the three RNA silencing suppressor genes of south american ctv isolates reveal the circulation of a novel genetic lineage. |
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Fecha de publicación : |
2015 |
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Fuente / Imprenta : |
Viruses, 2015, v.7, no.7, p.4152-4168. OPEN ACCESS. |
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ISSN : |
1999-4915 |
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DOI : |
10.3390/v7072814 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received: 26 May 2015 / Accepted: 17 July 2015 / Published: 22 July 2015. |
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Contenido : |
ABSTRACT.
Citrus Tristeza Virus (CTV) is the most economically important virus of citrus worldwide. Genetic diversity and population structure of CTV isolates from all citrus growing areas from Uruguay were analyzed by RT-PCR and cloning of the three RNA silencing suppressor genes (p25, p20 and p23). Bayesian phylogenetic analysis revealed the circulation of three known genotypes (VT, T3, T36) in the country, and the presence of a new genetic lineage composed by isolates from around the world, mainly from South America. Nucleotide and amino acid identity values for this new genetic lineage were both higher than 97% for the three analyzed regions. Due to incongruent phylogenetic relationships, recombination analysis was performed using Genetic Algorithms for Recombination Detection (GARD) and SimPlot software. Recombination events between previously described CTV isolates were detected. High intra-sample variation was found, confirming the co-existence of different genotypes into the same plant. This is the first report describing: (1) the genetic diversity of Uruguayan CTV isolates circulating in the country and (2) the circulation of a novel CTV genetic lineage, highly present in the South American region. This information may provide assistance to develop an effective cross-protection program. |
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Palabras claves : |
CITRUS TRISTEZA VIRUS; RT-PCR. |
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Thesagro : |
CITRUS; CRUZAMIENTO INTERCROMOSOMICO; FILOGENIA; RECOMBINACION; URUGUAY. |
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Asunto categoría : |
F30 Genética vegetal y fitomejoramiento |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/5167/1/Rivas-F.-Maeso-D.-2015.-Viruses.pdf
http://www.mdpi.com/1999-4915/7/7/2814
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Marc : |
LEADER 02270nam a2200289 a 4500
001 1053851
005 2019-10-28
008 2015 bl uuuu u0uu1 u #d
022 $a1999-4915
024 7 $a10.3390/v7072814$2DOI
100 1 $aBENÍTEZ-GALEANO, M.J.
245 $aPhylogenetic studies of the three RNA silencing suppressor genes of south american ctv isolates reveal the circulation of a novel genetic lineage.$h[electronic resource]
260 $aViruses, 2015, v.7, no.7, p.4152-4168. OPEN ACCESS.$c4168
500 $aArticle history: Received: 26 May 2015 / Accepted: 17 July 2015 / Published: 22 July 2015.
520 $aABSTRACT. Citrus Tristeza Virus (CTV) is the most economically important virus of citrus worldwide. Genetic diversity and population structure of CTV isolates from all citrus growing areas from Uruguay were analyzed by RT-PCR and cloning of the three RNA silencing suppressor genes (p25, p20 and p23). Bayesian phylogenetic analysis revealed the circulation of three known genotypes (VT, T3, T36) in the country, and the presence of a new genetic lineage composed by isolates from around the world, mainly from South America. Nucleotide and amino acid identity values for this new genetic lineage were both higher than 97% for the three analyzed regions. Due to incongruent phylogenetic relationships, recombination analysis was performed using Genetic Algorithms for Recombination Detection (GARD) and SimPlot software. Recombination events between previously described CTV isolates were detected. High intra-sample variation was found, confirming the co-existence of different genotypes into the same plant. This is the first report describing: (1) the genetic diversity of Uruguayan CTV isolates circulating in the country and (2) the circulation of a novel CTV genetic lineage, highly present in the South American region. This information may provide assistance to develop an effective cross-protection program.
650 $aCITRUS
650 $aCRUZAMIENTO INTERCROMOSOMICO
650 $aFILOGENIA
650 $aRECOMBINACION
650 $aURUGUAY
653 $aCITRUS TRISTEZA VIRUS
653 $aRT-PCR
700 1 $aRUBIO, L.
700 1 $aBERTALMIO, A.
700 1 $aMAESO, D.
700 1 $aRIVAS, F.
700 1 $aCOLINA, R.
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